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By searching for transcription factors that function at these elements, we identified NFIA and ATF3 as drivers of astrocyte differentiation from neural precursor cells while RUNX2 promotes astrocyte maturation. We report molecularly distinct phases of astrogliogenesis that exhibit stage- and lineage-specific transcriptomic and epigenetic signatures with unique primed and active chromatin regions, thereby revealing regulatory elements and transcriptional programs underlying astrocyte generation and maturation. Here we perform comprehensive and integrated transcriptomic and epigenomic analyses to delineate gene regulatory programs that drive the developmental trajectory from mouse embryonic stem cells to astrocytes. That it became one of the few chart-toppers from the mid-90s pop-reggae revival may be something of an accident of timing in that it took advantage of traditionally low sales at the turn of the year, but it's fortuitous because this proves to be one of the best examples of the 'cover an old song with some toasting over it' subgenre.A broad molecular framework of how neural stem cells are specified toward astrocyte fate during brain development has proven elusive. And of all the versions of this song, this is the only one ever to reach Number One. I think only Shaggy has matched them in this regard.

In minor contrast to the preceding track, I remember this one very well but didn't realise until much later how much other success Chaka & Pliers had had: this was their third consecutive UK Top 5 single, an unprecedented feat for a Jamaican act (though it was reportedly only a last-minute change of mind by the record company that awarded them lead credit). Also, I should point out that Blogger doesn't seem to like ampersands, so if any of the ones in this post go a bit haywire, apologies. Download Nada Dering Alarm Kebakaran here. It followed 'Tease Me' and 'She Don't Let Nobody' and has now been followed into the chart by 'Murder She Wrote' Before we start, I should acknowledge that yes, I am stretching a point somewhat by including the 'Beatles cover' tag down there, but I ultimately concluded that they were the act most commonly associated with this song even though theirs wasn't the original version and was never released as UK single - it finally made the Top 50 in 2010 when it was made available as a download. Chart Peak: 1 (2 weeks) 'Twist And Shout', a chart-topper in January 1994, was the 3rd of the duo's 4 UK hits to date.